Toxoplasma genotyping among infected human and animal hosts using PCR-restriction fragment length polymorphism: study in Al-Madinah, Saudi Arabia
واصفات البياناتعرض سجل المادة الكامل
The progression and severity of Toxoplasma disease differ due to several variables, including host and parasite genetics. Exposing the possible correlation between the genotypes and the severity of the disease might be of value to identify the outcomes of the infection and hence, the proper management of infected cases. This study was conducted to identify the possible lineage type of Toxoplasma from blood samples of humans and animals for the first time in Al Madinah, Saudi Arabia. Methods Preliminary detection of Toxoplasma B1 gene was done by a nested PCR. Subsequent identification of Toxoplasma strain was done for 91 positive samples using PCR- Restriction fragment length polymorphism (RFLP) at the SAG2 loci of T. Gondii and restriction enzymes HhaI and Sau3AI. Results Type II isolate was found to be the predominant one (45.1%). The other genotypes I and III were detected in 20.9% and 21.9% of cases respectively. The remaining 11 cases (12.1%) were of unknown genotypes. In general, there was no clear correlation between strain genotype and the different clinical forms. Except for genotype III which was not detected among the 7 congenitally infected cases, all the three classical types and the unknown one were detected in all clinical forms Conclusion It remains uncertain to what extent the genotype of the parasite directly contributes to the clinical severity of human toxoplasmosis. Further studies are recommended, using larger number of samples, especially in cases of congenital toxoplasmosis infection where treatment might be improved.